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Tuesday, 10 January 2012

10. Function of restriction II enzyme


    A.  Prevents protein  folding
    B.  Removing  formed DNA
    C.  Cleaves DNA  at palindromic recognition site
   D.   Negative supercoiling
Answer isRestriction enzymes Cut at Palindromic Sequences

Structure and function of type II restriction endonucleases

Abstract

More than 3000 type II restriction endonucleases have been discovered. They recognize short, usually palindromic, sequences of 4–8 bp and, in the presence of Mg2+, cleave the DNA within or in close proximity to the recognition sequence. The orthodox type II enzymes are homodimers which recognize palindromic sites.[http://nar.oxfordjournals.org/content/29/18/3705.full]

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